The CMO ORF was cloned and the plant expression vector pBI121-CMO was constructed. The resultant plasmids pBI121-CMO was transferred into Agrobacterium tumefaciens (GV3101) by the liquid nitrogen freezing thaw method.
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- 克隆了CMO基因的編碼區,構建了其植物表達載體pBI121-CMO,凍融法將質(zhì)粒導入根癌農桿菌(Agrobacterium tumefaciens)GV3101,獲得工程菌GV3101-pBI121-CMO。