Silver staining was fit to ascertain protein spot in 2-DE gels, coomassie blue was fit to detect high content protein, DIGE was fit to establish difference protein expression map.

 
  • 在三種蛋白質(zhì)的檢測方法中,硝酸銀染色適合確定蛋白質(zhì)點(diǎn)的有無(wú),考馬斯亮藍R-250染色適合目標蛋白質(zhì)為高豐度且樣品量大的情況,DIGE技術(shù)適合用于差異蛋白質(zhì)表達譜的分析。
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