Shim-pack VP-ODS is used as column, ACN: EtOAc(21:3, V/V) as mobile B, and aqueous acetic acid (50:1,V/V) as mobile A, linear gradient elution is adopted.

 
  • 流動(dòng)相A為2%25醋酸(HAc),流動(dòng)相B為乙腈(ACN); 乙酸乙酯(EtOAc)=21:3(v/v),梯度洗脫;
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