SPC-A1 cells were inbucated with the pharmaceutical serum containing Hua Tan San Jie Fang and rabbit serum not containing drug separately for 12h,24h,36h,48h,then the expression of TNFR1 mRNA was examined by in situ hybridzation method.

 
  • 化痰散結方含藥血清,不含藥兔血清分別與人肺癌細胞SPC-A1共同孵育12小時(shí)、24小時(shí)、36小時(shí)、48小時(shí)后,運用原位雜交的方法檢測人肺癌細胞TNFR1表達的變化。
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