Renalembryo 293 cell was co-transfected with the rAAV vector of plasmid pSSHG/NT4-GFP-Ant ,packaging plasmid pAAV/ Ad and helper adeno virus pasmidpFG140.The recombinant AAV viral stock was titrated by dot blot.
英
美
- 用腺病毒輔助質(zhì)粒Pfg140、包裝質(zhì)粒pAAV/Ad及已構建的重組腺伴隨病毒載體質(zhì)粒,三質(zhì)粒磷酸鈣共沉淀法轉染80%25融合的293細胞系,包裝rAAV。
