The secretion of recombinant protein to periplasm or extracellular medium has more advantages than the expression intracellular as inclusion bodies. 重組蛋白分泌到周質(zhì)空間或胞外培養基中較之在胞內以包含體形式表達有許多優(yōu)勢。
Mice were used to determine the hypocalcemic effect of the recombinant protein.Results showed that the recombinant protein expressed in E.coli had the proper bioactivity. 利用小鼠降血鈣實(shí)驗檢測變性復性后產(chǎn)物的活性，結果表明該重組蛋白有一定的生物活性。
