Recently, the J6-1 membrane associated factor, stimulating proliferation of J6-1 cells, was discovered and designed as MAF-J6-1. After fractionation via Sephadex G-100 and sequential two steps FPLC Mono Q, it was purified over 333-fold.
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- 經(jīng)Sephadex G-100凝膠過(guò)濾及連續兩次快速蛋白液相層析(FPLC,Mono Q)將其分離。