RT-PCR was used to estimate the relative mRNA amounts of estrogen receptor(ER),MTA3 and snail in the cells. Western blotting and gelatin zymography assay were used to estimate the relative protein amounts of MMP-2,MMP-9 and E-cadherin in the cells.

 
  • 采用RT-PCR技術(shù)檢測雌激素受體(ER)以及與侵襲有關(guān)的MTA3、Snail基因mRNA的表達,采用蛋白印跡法和明膠酶譜法檢測E-鈣黏附蛋白(E-cadherin)和基質(zhì)金屬蛋白酶2(MMP-2)和9(MMP-9)蛋白的表達。
今日熱詞
目錄 附錄 查詞歷史
国内精品美女A∨在线播放xuan