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- 合成的方案是采用先合成AGD、CGG和DAG三個(gè)三核苷二磷酸片段,然后再以AGD+pCGGDAG(3+p6)或AGDCGG+pDAG(6+p3)兩種方式用T_4 RNA連接酶連接成為所需要的九核苷酸。As the first step, three trinucleoside diphosphate fragments AGD, CGG and DAG were synthesized and then, in the presence of T_4 RNA ligase, joined to form the desired nonamer either through route 3+ p6 (AGD+ pCGGDAG) or route 6+p3 (AGDCGG+pDAG). The phosphodiester methed was used in the syntheses of CGG and DAG;
- 泛素連接酶ubiquitin ligase, ubiquitinligating enzyme
- 輔酶A連接酶CoA ligase
- 酶的enzymatic
- 泛素連接酶E3Ubiquitin ligase E3
- 輔酶coenzyme
- 酶解zymohydrolysis
- 過(guò)氧化氫酶catalase
- 纖維素酶cellulase
- 碳氮連接酶類(lèi)Carbon-nitrogen ligases
- 酶活性enzyme activity
- 酶學(xué)enzymology
- 堿性磷酸酶alkaline phosphatase
- E3泛素連接酶E3 ligase
- 連接酶合成產(chǎn)物的瞬時(shí)速度與細胞中可利用的ATP含量成比例。The degree of bond formation by ligases is proportional to the amount of ATP available in the cell at a particular instant.
- 方法載體的構建采用限制性?xún)惹忻该盖小?DNA連接酶連接等方法。Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid.
- 氨基酸RNA連接酶amino acid REN ligase
- 氨基酸核糖核酸連接酶amino-acid-RNA ligase
- 醛連接酶carboligase
- DNA連接酶DNA ligase; DNA sealase; polydeoxyribonucleotide synthase
