Plaque assays were done to isolate those white recombinant clones for rhBMP2 and 3. PCR analyses of viral DNAs were done to identify the target DNAs in recombinant viruses .

 
  • 空斑形成后,經(jīng)X-gal染色,挑取白色的單個(gè)重組克隆保存,取B2和B3各5個(gè)白色克隆進(jìn)行第一輪擴增,收集病毒上清。
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