Original strain, Corynebacterium glutamicum AS1542, was mutanted by DES and UV. protoplast irradiation. Finally, a higher L-tryptophan producing strain,T11-6 was obtained. It was a double auxotroph of phenylalanine and tyrosine.
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- 以谷氨酸棒桿菌AS1542為出發(fā)菌株，經(jīng)過(guò)硫酸二乙酯誘變和紫外原生質(zhì)體誘變，定向選育出一株穩定的色氨酸生產(chǎn)菌T11－6，該菌株具有苯丙氨酸、酪氨酸雙重營(yíng)養缺陷及對氟苯丙氨酸、5-甲基色氨酸、6-氟色氨酸三種結構類(lèi)似物抗性遺傳標記。
