Optimized-code bull protamine gene was synthesized, the code (CGT) which E. coli. is partial to was substituted for the rare codes(AGA or AGG), the gene was cloned into pGEX-2T to construct expression vector pGEX-2T-PS.
英
美
- 利用大腸桿菌偏好的編碼精氨酸的密碼子CGT將野生型牛精蛋白基因中編碼精氨酸的稀有密碼子 (AGA或AGG)替換掉 ,通過(guò)基因合成得到密碼子優(yōu)化的牛精蛋白基因 ,將其克隆入原核表達載體pGEX 2T ,組裝成表達載體pGEX 2T PS。
