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- The mRNA expression of ubiquitin in rat EDL muscle was determined by northern blot analysis. 應用northernblot方法測定伸指長(cháng)肌組織泛素mRNA的表達變化 ,分析骨骼肌蛋白降解與泛素mRNA表達的關(guān)系。
- Kumark Saivithriy S. MRNA levels of Ca2+-independent form of protein kinase C in poslinchemic gerbil brain by northern blot analysis [J],J Biol Chem, 1997,252(61):7603. 盧布峰;魯友明;黃詒森;蛋白激酶C對大鼠腦缺血海馬突觸體谷氨酸攝取的調控作用[J].;生物化學(xué)雜志;1994;10(3):371
- We obtain 15 differentially expressed genes to human glioma thruogh four times hybridizations and scanning. Northern blot analysis confirmed 436F11 clone was over expression in human brian tissue and low expression in human glioma tissues. 通過(guò)四次基因芯片篩選 ,獲得 15條與膠質(zhì)瘤相關(guān)的新基因 ,經(jīng)northernblot證實(shí) 436F11基因在人正常腦組織中高表達 ,而在人腦膠質(zhì)瘤中低表達。
- The expression of the novel gene in 12 specimens of pancreatic cancer and normal tumor-surrounding pancreas tissues and 4 pancreatic cancer cell lines(PaTu8988,SW1990,BxPC-3,AsPC-1)were detected by RT-PCR and Northern blot analysis. 應用RT-PCR和Northernblot檢測新基因在12例胰腺癌和癌旁正常胰腺組織以及4種胰腺癌細胞株(PaTu8988、SW1990、Bx-PC-3、AsPC-1)中的表達情況。
- Improvements on the Methods of Plant RNA Isolation and Northern Blot Analysis, and Effects of Plastid Signal on Nuclear Gene Expression as Well as Leaf Morphogenesis 植物RNA分離與Northern雜交方法的改進(jìn)及質(zhì)體信號對核基因表達和葉片形態(tài)建成的影響
- Methods Northern blot analysis was used to study the expression of c myc and c fos m RNA in human embryo lung(HEL)cells exposed to cigarette smoking solution(CSS) and chrysotile(CH)separately or simultaneously; 方法 用Northernblot測定了煙溶液 (CSS)與溫石棉 (CH)單獨及聯(lián)合作用后人胚肺 (HEL)細胞c fos和c myc癌基因mRNA水平的表達。
- Blast analysis displayed one of the Northern blot positive clone is homologous with autotaxin gene, and another is homologous with calcyclin gene. 對這13個(gè)克隆進(jìn)行測序和Blast同源序列分析表明,其中一個(gè)Northern雜交陽(yáng)性克隆與autotaxin基因同源,另一個(gè)與鈣周期蛋白基因同源。
- Northern and Western blot analysis in pMR-FG1 and pBI-FG1transformants showed that pMR-FG1 can improve the gene expression both intranscription level and in translation level. 對部分 pMR-FG1 和 pBI-FG1 轉化植株的 Northern blot 和 Westernblot 分析證明,pMR-FG1 轉化植株中融合蛋白的表達在轉錄水平和翻譯水平明顯高于 pBI-FG1 轉化植株。
- This CTB gene product was also verified by Western blot analysis. Western blotting結果確認了該條帶為CTB基因的產(chǎn)物。
- The results of western blot analysis were in accordance with those of 2-DE. 對部分差異蛋白采用Western-blot方法在蛋白水平的驗證結果與2-DE結果基本相符。
- Northern blot showed that PBP-Harm and GOBP2-Harm are specifically expressed in the antenna of H.armigera. Northern雜交結果表明:PBP-Harm和GOBP2-Harm都在棉鈴蟲(chóng)觸角中特異性表達。
- PAI-1 protein secretion by mesangial cells was measured by Wes tern blot and PAI-1 mRNA by both RT-PCR and Northern blot. RT-PCR和Northern雜交檢測PAI-1mRNA表達;
- With RT-PCR and Northern blot methods, the expression patterns of mNTKL-BPl were observed in the normal tissues of mouse. 用RT-PCR和Northern blot方法觀(guān)察到mNTKL-BP1基因在小鼠的正常組織中均有表達。
- Transgenic potato plants 1M, 2M, 3M, 5M, 10M, llM provided by laboratory were analyzed by Southern blot and Northern blot. ELISA檢測轉基因馬鈴薯病毒抗性數據顯示,溫室條件下,3個(gè)轉基因馬鈴薯株系(2M、3M、11M)具有病毒抗性,3個(gè)株系(1M、5M、10M)感??;
- Northern blot -- An electroblotting method in which RNA is transferred to a filter and detected by hybridization to32 P-labelled RNA or DNA. 北方轉漬法--一種電子轉印方法,將RNA轉移至濾紙上,加上要試驗的放射性標志RNA或DNA,以進(jìn)行雜合,以自動(dòng)顯影技術(shù)觀(guān)察
- RT-PCR, Western blot analysis and FACS were used to detect the expression of P2X7 receptor in these clones. 熒光分光光度計檢測P2X7受體介導的胞內鈣離子濃度變化。
- Using morphology observation, the RT-PCR and Western blot analysis to investigate and analysis the sub-type of CnA. 通過(guò)病理形態(tài)學(xué)檢查、RT-PCR實(shí)驗測定及Western blot分析確認CnA亞型。
- Animals were killed at day 3,7,14and 28 after BLM administration. The total RNA was extracted from the lung tissue. The expressions of SPsmRNA were analyzed with Northern blot. 方法氣管內灌注博萊霉素A5,復制肺纖維化模型,分別于3、7、14和28天處死動(dòng)物,提取肺組織總RNA,進(jìn)行Northern雜交。
- The samples were examined delicately to investigated the expression of LeY oligosaccharide by Western blot analysis. 應用Western免疫印跡法,觀(guān)察Ley寡糖抗原的表達。
- Expression of messenger endothelin 1 (ET 1) RNAs and its receptor subunits (ETA,ETB)were studied by northern blot hybridization in rat kidney following renal ischemia reperfusion. 為了探究?jì)绕に?1(ET?1)對腎功能的影響和作用方式,采用斑點(diǎn)雜交和原位雜交方法對大鼠缺血60分鐘再灌注腎組織ET?1及其受體亞型(ETA、ETB)的基因表達進(jìn)行了研究。