MethodsThe optimized host cell,culture medium and induction time were deternined according to the growth and expression specificity of nrhTNF on test tube and flask shaker. Then fed batch culture was carried out on 5 L automatic fermentor.
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- 方法通過(guò)探討n(yōu)rhTNF工程菌在試管和錐形搖瓶中的生長(cháng)和表達規律 ,篩選并確定其最適宿主菌、最佳培養基及最佳誘導表達條件 ,然后在 5L自控發(fā)酵罐中進(jìn)行分批補料培養。
