Methods\ Each superovulated female of 615 mice was placed individually with a stud male and was checked the next morning.

 
  • 方法采用經(jīng)超量排卵的615雌鼠與正常雄鼠交配,取受精卵,再經(jīng)手術(shù)分別將單胚卵、雙胚卵移入發(fā)情的假孕雌鼠的輸卵管中,妊娠產(chǎn)仔。
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