Methods:The assay was based on precolumn derivatization of cystine(Cys)with 2,4-dinitrofluorobenzen(DNFB) in the phosphate buffer system(pH9.5),the quantitative analysis was performed with external standard under the optimal conditions.
英
美
- 方法:在堿性條件下;2;4-二硝基氟苯(DNFB)與胱氨酸反應生成 DNFB 氨基酸衍生物;在四硼酸鈉緩沖液(pH 9.;8)中電泳分離;電壓28kV;檢測波長(cháng)340nm。