Methods:The Descemet s membrane with its endothelial covering was separated from the underlying stroma by microdissection.

 
  • 方法:顯微分離兔角膜后彈力層-內皮細胞層,采用貼壁法、貼壁消化法及兩種消化法培養,觀(guān)察每種方法細胞生長(cháng)的特點(diǎn)。
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