Methods:The 293T cells was cotransfected with PDG and an AAV plasmid containing human apoAIcDNA to produce infectious rAAV, and it was purified by non ionic iodixanol gradients followed by heparin affinity chromatography.
英
美
- 方法 :以質(zhì)粒 PDG和含有人 apo AIc DNA的AAV載體共轉染 2 93T細胞 ,繼以 Iodixanol密度梯度離心結合肝素親和層析法分離、純化 ,斑點(diǎn)雜交鑒定 r AAV病毒顆粒數 ;
