Methods:NMPs were prepared by high salt extraction and immunized rabbit to raise polyclonal antibody which was used to detect the membrance transferred from 2 D gel of nuclear matrix in normal WBC and acute myeloid leukemia cells by Western blot.

 
  • 方法 :高鹽抽提法制備白血病細胞NMPs ,免疫動(dòng)物制備多克隆抗體 ,以此作為免疫學(xué)探針對正常WBC和白血病細胞NMPs進(jìn)行雙向電泳 ( 2DE)_免疫印跡 (Westernblot)檢測。
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