Methods:Mouse fibroblast NIH3T3 cells were transfected with 3 2 kb HBV DND by exposure of the cells to calcium phosphate.

 
  • 方法:應用磷酸鈣轉染技術(shù)構建表達HBsAg和HBeAg的小鼠NIH3T3細胞克隆,以該細胞為模型,應用原位雜交技術(shù)測定細胞內特異性TIMPRNA含量變化。
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