Methods:Melittin was isolated and purified from bee venom by gel filtration on Sephadex G 25,Sephadex G 50 and Sephadex G 75.Homogenicity and molecular weight of melittin were determined by SDS PAGE and HPLC.
英
美
- 方法 :用 Sephadex G- 2 5、Sephadex G- 5 0、Sephadex G- 75三步層析法從蜜蜂毒中分離純化蜂毒素 ,以SDS- PAGE定性 ,以 HPL C進(jìn)行含量測定。
