Methods:Cell culture technique, MTT colorimetric assay, ALP activity assay, Commasie brilliant blue stain and Dahl McGec Russell s alizarin red stain for calcium were used.

 
  • 方法:采用細胞培養、MTT比色測定法、堿性磷酸酶(ALP)測定法、考馬斯亮藍法和茜素紅染色法。
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