Methods:Because of the overlapping gene between trpE and trpD,the trpED was cloned integrallty to produce a new high-expression vector pBV220-trpED.

 
  • 由于trpE和trpD存在翻譯偶聯(lián)現象,將其自大腸桿菌基因組中經(jīng)PCR擴增得到,構建新的表達質(zhì)粒pBV220-trpED。
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