Methods pVAX1-GRA8 was purified and transfected into Africa green monkey kindey vero cell lines,expression of GRA8 in vero cell lines were detected by RT-PCR and immunoblot with rabbits sera infected with Toxoplasma gondii.
英
美
- 方法:大量提取純化重組真核表達質(zhì)粒pVAX1-GRA8,瞬時(shí)轉染培養的vero E-6細胞,RT-PCR和免疫印跡法檢測GRA8在細胞中的表達。
