Methods Whole cell patch clamp techniques were employed to investigate the effect of aminophylline on L-type calcium cur-rent of diaphragmatic muscles in rats.
英
美
- 方法:采用全細胞膜片鉗（whole cell patchclamp）技術(shù)；當維持電位為-80mv；刺激頻率為0.；5Hz；鉗制時(shí)間為300ms；步進(jìn)電壓為10mv；去極到60mv；觀(guān)察氨茶堿對膈肌細胞L型鈣通道（L-ICa）的影響。
