Methods Total RNA extracted from rat brian cell was transcripted reversely into cDNA with random primers. The Glutamic acid decarboxylase 67 gene fragments were amplified using PCR method and sequenced using the dideoxy chain termination technique.
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- 方法 采用RT -PCR方法 ；大鼠腦組織中的mRNA逆轉錄成cDNA ；再以cDNA為模板 ；擴增谷氨酸脫羧酶GAD6 7基因片段 ；克隆入T載體 ；并經(jīng)序列測定 .
