Methods The probe of PNA and DNA were immobilized on the gold surface with bioth avidin method,and hybridized with target sequence of the complete,one mismatch and two mismatch sequence,respectively.
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- 方法用生物素親和素法分別將PNA、DNA兩種探針固定在基因傳感器的金膜表面,分別與完全匹配、1個(gè)堿基錯配、2個(gè)堿基錯配的互補靶序列進(jìn)行雜交,觀(guān)察2種不同的探針與靶序列反應所引起的頻率變化及所用的時(shí)間。
