Methods The growth effects of EGF and NC on U251 MG cultured in serum-free and serum containing (10% fetal bovine serum, FBS) media respectively were observed by MTT colorimeritric analysis.

 
  • 方法 :在含 10%25小牛血清和無(wú)血清培養基中 ,加入不同濃度的EGF ,NC及NC和EGF(10ng·ml-1)干預 ,應用MTT法觀(guān)察其細胞生長(cháng)效應。
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