Methods The expression of Survivin protein and gene was detected by SP immunohistochemical technique and RT-PCR in 48 cases of cholangiocarcinoma,30 cases of adjacent histologically noncancerous bile duct respectively.

 
  • 方法應用免疫組化SP法檢測48例膽管癌和其中30例癌旁膽管組織中Survivin蛋白的表達,同時(shí)應用RT-PCR技術(shù)檢測這些組織標本中Survivin mRNA的表達,并與臨床病理資料進(jìn)行相關(guān)性分析。
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