Methods The CHO/dhfr- cells were transfected with linearized plasmids of pMM-CTLA-4-IgG4/WG and pMMGR. The clones of CHO/pCTLA-4+/pMMGR+ were in suspension cultured in serum-free culture media.
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- 方法將線(xiàn)性化的pMM-CTLA-4-IgG4/WG和pMMGR轉染CHO/DG44細胞,對陽(yáng)性克隆進(jìn)行無(wú)血清懸浮培養,Western blot檢測細胞培養液中CTLA-4/Ig融合蛋白的表達,篩選出高效穩定表達CTLA-4/Ig融合蛋白的CHO細胞;
