Methods Survivin-targeting shRNA eukaryotic expression vector pRNAT-U6. 1/neo-survivin was constructed and identified. Human bladder cancer cell T24 was transfect-ed with pRNAT-U6. 1/neo-survivin and then stable monoclonal cells were selected.
英
美
- 方法構建Survivin基因shRNA真核表達載體pRNAT-U6.;1/neo-survivin;轉染人膀胱癌細胞T24并獲得穩定的單克隆細胞。