Methods Survivin-targeting shRNA eukaryotic expression vector pRNAT-U6. 1/neo-survivin was constructed and identified. Human bladder cancer cell T24 was transfect-ed with pRNAT-U6. 1/neo-survivin and then stable monoclonal cells were selected.

 
  • 方法構建Survivin基因shRNA真核表達載體pRNAT-U6.;1/neo-survivin;轉染人膀胱癌細胞T24并獲得穩定的單克隆細胞。
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