Methods Recombinant expression vectors containing CD (cytosine deaminase)and/or TK(thymidine kinase) gene under CMV promoter were constructed successfully. The vectors were transfected to GLC-82 tumor cell lines by use of lipofectamine.
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- 方法 分別構建了以 CMV為啟動(dòng)子 ；含單純皰疹病毒胸苷激酶（ HSV-TK）和 /或大腸桿菌胞嘧啶脫氨酶（ Ecoli.；CD）單、雙自殺基因的真核表達載體。
