Methods RNA was isolated from the human osteosarcoma cell line U-20S by single-step method of acid guanidinium isothiocyanate phenol-chloroform extraction.

 
  • 方法 采用異硫氰酸胍一步法從人骨肉瘤細胞株U-2OS 中提取總RNA,以逆轉錄一聚合酶鏈反應(Reverse Transcription coupled Polymerase Chain Reaction,RT-PCR)擴增cDNA基因片段。
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