Methods Polymerase chain reaction (PCR) based microsatellite polymorphism analyses were performed to detect LOH on chromosome 13,fluorescence labeled primers and Perkin Elmer 377 DNA Sequencer were applied.

 
  • 方法 應用聚合酶鏈反應 (PCR)方法 ,采用熒光標記的引物和 377型DNA序列自動(dòng)分析儀 ,分析了 2 0例GBM 13號染色體上 14個(gè)微衛星多態(tài)性標記的LOH。
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