Methods Plasmids including pDOR-neo vector,pDORHRAD17s(sense) and pDOR-HRAD17a(anti-sense) were transfected into mouse fibroblasts(NIH3T3) and human uterine cervix cancer cells(HeLa).
英
美
- 方法利用基因轉染技術(shù),對小鼠成纖維細胞NIH3T3和人宮頸癌HeLa細胞均分別轉染pDOR-neo載體,pDOR-HRAD17s(正義)和pDOR-HRAD17a(反義)質(zhì)粒,G418篩選獲得陽(yáng)性克隆。
