Methods Mononuclear cells were isolated from human peripheral blood cells by density gradient centrifugation and cultured in M199 medium with fetal blood serum(FBS),vascular endothelial growth factor(VEGF) and basic fibroblast growth factor(bFGF).
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- 方法用密度梯度離心法從人外周血中分離單個(gè)核細胞。 在加有胎牛血清(fetal blood serum,FBS)、血管內皮生長(cháng)因子(vascular end othelial growthfactor,VEGF)和堿性成纖維生長(cháng)因子(basic fibroblast growthfactor,bFGF)的M199培養液中培養。
