Methods Mice were immunized with bovine submaxillary mucin (BSM), the splenic lymphocytes from the immunized mouse were fused with the mouse myeloma Sp2/0 cells using PEG.

　
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  • 方法 以牛頜下腺粘蛋白(BSM)為免疫原免疫小鼠,以免疫脾細胞與Sp2/0細胞融合建立雜交瘤細胞株,用有限稀釋法克隆化,間接ELISA及間接ELISA結合神經(jīng)氨酸酶消化篩選克隆。
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