Methods Lipofectin method was used to transfect pCR3.1/CCK_2R vector expressing gastrin receptor into a colon cancer cell line col0320.Gastrin and gastrin antagonist were used to up-regulate and down-regulate the signaling pathway,respectively.
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- 方法采用脂質(zhì)體介導法;將胃泌素受體(CCK_2R)的真核表達載體pCR3.;1/CCK_2R穩定轉染人結腸癌細胞株colo320;上調胃泌素的作用;