Methods KG_1 was stimulated by phorbol ester(PMA) in combination with ionomycin. Expression of DC-SIGN was detected by western blotting, RT-PCR, and flow cytometry in KG_1 and KG_1 derived DLCs of different stimulation periods.

 
  • 方法采用佛波酯(PMA)和離子霉素(Ionomycin)誘導KG1細胞為DLCs,采用Western免疫印跡、RT-PCR和流式細胞儀檢測KG1和KG1來(lái)源的DLCs不同誘導時(shí)期DC-SIGN的表達。
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