Methods Immunohistochemistry and in situ hybridization technique were used to detect DCN protein and mRNA expressions in 30 cases of colorectal carcinoma tissues and 20 cases of relative normal tissues.

 
  • 方法:采用原位雜交及免疫組織化學(xué)方法檢測DCN mRNA及其蛋白在30例結直腸癌及20例相對正常大腸組織中的表達。
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