Methods Genomic DNA was isolated with kit, and the mitochondrial NADH dehydrogenase 1(ND1) and cytochrome c oxidase I (COI) gene fragments were amplified by polymerase chain reaction(PCR) and sequenced.
英
美
- 方法 試劑盒抽提基因組總DNA后 ,以特異性引物對線(xiàn)粒體還原型煙酰胺腺嘌呤二核苷酸 (NADH)脫氫酶 1(ND1)和細胞色素c氧化酶I(COI)進(jìn)行PCR擴增 ,將PCR產(chǎn)物分別測序 ,并以生物信息學(xué)方法加以比較 ,構建系統進(jìn)化樹(shù)。
