Methods 20 loci on chromosome 9 were examined to detect loss of heterozygosity(LOH) in 21 cases of glioblastoma by PCR based microsatellite polymorphism analy-ses, in which fluorescence-labeled primers and Perkin Elmer 377 DNA Sequencer were applied.

 
  • 方法 應用聚合酶鏈反應(PCR)方法,采用熒光標記引物和377型DNA序列自動(dòng)分析儀,分析21例 GBM 9號染色體上 20個(gè)微衛星多態(tài)性標記的LOH。
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