Method:Trypan blue exclusion, 3H|TdR incorporation and 3H|proline incorporation were assayed when LFb cultured in AM|conditioned medium on day 7 after bleomycin exposure. Western blot was carried out to determine the change of Ras process.
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美
- 方法 :肺纖維化 1周組肺泡巨噬細胞 (AM)條件培養上清液與正常大鼠LFb共同培養 ,并給予不同濃度的Sim干預 ,利用3H 胸腺嘧啶核苷摻入法、3H 脯氨酸摻入法和Western雜交測定LFb增殖、膠原合成和Ras蛋白的翻譯后修飾。
