Method: (1) NS3 protease gene and single chain NS3/4A gene were both subcloned into pQE-30 and expressed in E.

 
  • 方法:(1) 用原核表達載體pQE-30分別構建含有NS3蛋白酶基因的pQE/NS3和含有單鏈NS3/4A基因的pQE/NS3/4A重組質(zhì)粒;
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