METHODS: The eukaryotic expression vector pcDNA3.0 containing human full length HAb18G cDNA and empty vector pcDNA3.0 were introduced respectively into NIH/3T3 fibroblast cells by liposome mediated gene transfection method.
英
美
- 方法 :將含有肝癌相關(guān)抗原HAb1 8G全長(cháng)cDNA的真核表達載體 pcDNA3.；0 /HAb1 8G利用脂質(zhì)體介導法轉染小鼠成纖維細胞NIH/ 3T3；G4 1 8篩選建立穩定表達陽(yáng)性克隆細胞株t3T3; 利用流式細胞儀、間接免疫熒光染色方法鑒定目的蛋白轉染情況 ;
