METHODS: Eukaryotic expression vectors pTL 8/apical membrane antigen 1 (AMA 1) (tTA) and pTL 8/AMA 1(rtTA)gene which express trans activator (tTA) or reverse trans activator(rtTA), respectively, and AMA 1 gene of Plasmodium falciparum were constructed.
英
美
- 方法 :首先構建惡性瘧原蟲(chóng)頂端膜抗原 1(AMA 1)基因和轉錄激活因子 (tTA或rtTA)基因的真核表達質(zhì)粒pTL 8/AMA 1和pTL 8/AMA 1(rtTA) ,并大量制備這兩種質(zhì)粒及表達轉錄抑制子 (tTS)的質(zhì)粒pUHS6 1。
