METHODS Recombinant eukaryotic expression vector pcDNA3.1 S0.7 was constructed by cloning S segment 0.7 kb fragment into pcDNA3.1 (+) and transfected COS 7 cells by lipofectin. Immunoflourescence was used to identify the expression.

 
  • 方法 構建漢坦病毒 76 - 118株 S基因 5 端 70 0 bp片段的真核表達載體 pc DNA3.;1- S0
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