METHODS :The entire coding reg ions ,the splice donor /acceptor sites,and partial reg ulator y reg ions of SEMA3B and SEMA3F g ene were screened for mutations by P CR-sequencing in 21primary NPC tumors and 2NPC cell lines (CNE2and SUNE1).

 
  • 方法:采用PCR-直接測序方法,在21例散發(fā)性NPC組織和2例NPC細胞株(CNE2,SUNE1)中對SEMA3B和SEMA3F基因的編碼區、拼接位點(diǎn)和部分調控區進(jìn)行突變檢測;
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