METHOD: We extracted the RNA of Toxoplasma gondii (RH strain)by using a single step acid guanidinium thiocyanate method and isolated the poly(A) +RNA by using messenger RNA affinity membrane containing poly U to synthesize ds cDNAs.
英
美
- 方法：用異硫氰酸胍酸性一步法提取弓形蟲(chóng)（RH株）RNA，含PolyU的mRNA親和膜分離Poly（A）＋RNA，進(jìn)而合成雙鏈cDNA。
