Linoleate isomerase(LI ) gene was amplified by PCR from chromosome of Lactobacillus reuteri PYR8,and was inserted into pET30a to construct the prokaryotic expression vector pET30a-LI.
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- 以L(fǎng)actobacillus reuteri PYR8菌株基因組為模板,利用PCR方法擴增出亞油酸異構酶(linoleate isomerase,LI)基因,亞克隆到pET30a中構建原核表達載體pET30a-LI,并轉化入大腸桿菌(Escherichia coli)BL21(DE3)中。
